Table 1.

Reverse and forward primers for PCR cloning

Name	Target gene	Nucleotide sequence (5′–3′)	Length (bp)	Size of PCR product
P1 forward primer	Spike/S1	5′‐CCTCTCTCAGAAGCTAGCTGTACGTTGAA‐3′	29	819 bp
P1 reverse primer		5′‐ACGGACGGATCCTTAAGTAGTGTCAGCA‐3′	28
P2 forward primer	Spike/S2	5′‐TTAGCGGGTACAATCGCTAGCGGTTGGACCTTTGG‐3′	35	489 bp
P2 reverse primer		5′‐ ACTGAGGGAAGGAGAGCTCTTAATAGCCCTTTCC‐3′	34
N forward primer	Nucleocapsid	5′‐CTGAGATCACCGGCTAGCATGTCTGATAATGG‐3′	32	1257 bp
N reverse primer		5′‐ATGTCTGGCCAGGGATCCTTAGGCCTGAGTT‐3′	31

Abbreviation: PCR, polymerase chain reaction.