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. 2022 Nov 25;10(12):e735. doi: 10.1002/iid3.735

Figure 4.

Figure 4

Recombinant HSP40 (rHPS40) activated dendritic cells (DCs) via toll‐like receptor 4 (TLR4)‐dependent p38 mitogen‐activated protein kinase (p38 MAPK) and c‐Jun N‐terminal kinase (JNK) signaling pathways. (A) The activation of DCs by rHSP40 was TLR4‐dependent. PGN, peptidoglycan. Analysis of variance (ANOVA) was followed by Tukey's post hoc test: n = 3, versus group “shNC,” ns no significance, **p < .01, ***p < .001. (B) The activation of DCs by rHSP40 was inhibited by p38 MAPK and JNK inhibitors. ANOVA followed by Tukey's post hoc test: n = 3, ns, no significance; *p < .05, ***p < .001. (C) rHSP40 treatment increased phosphorylated levels of p38 and JNK. Left panel: Representative image of western blot analysis. Right panel: Statistical quantification based on the optical intensity of bands. ANOVA followed by Tukey's post hoc test: n = 3, versus group “0 min,” ns, no significance, **p < .01, ***p < .001.