Table 1.
Primers for plasmid construction and RT‐qPCR
| Primers | Sequence (5′→3′) |
|---|---|
| IL12A‐QF | ACCTCTTTCATAACTAATGG |
| IL12A‐QR | ACATCTTCAAGTCTTCATAA |
| IL12B‐QF | GTTGGTCATCTCTTGGTT |
| IL12B‐QR | GACATAAACATCTTTCTTCAGT |
| IL23A‐QF | AGATGAAGAGACTACAAATGAT |
| IL23A‐QR | AAGCAGAACTGACTGTTG |
| IL10‐QF | GCCTTTAATAAGCTCCAA |
| IL10‐QR | TTCGTATCTTCATTGTCAT |
| IL1B‐QF | CTTCAGCCAATCTTCATT |
| IL1B‐QR | ATTGCCACTGTAATAAGC |
| IL6‐QF | GGATTCAATGAGGAGACTT |
| IL6‐QR | ATCTGTTCTGGAGGTACT |
| TNFa‐QF | CAACCTCTTCTGGCTCAA |
| TNFa‐QR | TGGTGGTCTTGTTGCTTA |
| shTLR2‐F | CCGGGCATCTGATAATGACAGAGTTCTCGAGAACTCTGTCATTATCAGATGCTTTTT |
| shTLR2‐R | AATTAAAAAGCATCTGATAATGACAGAGTTCTCGAGAACTCTGTCATTATCAGATGC |
| shTLR4‐F | CCGGCCGCTGGTGTATCTTTGAATACTCGAGTATTCAAAGATACACCAGCGGTTTTT |
| shTLR4‐R | AATTAAAAACCGCTGGTGTATCTTTGAATACTCGAGTATTCAAAGATACACCAGCGG |
Abbreviations: F, forward primer for plasmid construction, QF, forward primer for RT‐qPCR; QR, reverse primer for RT‐qPCR; RT‐qPCR, reverse‐transcription quantitative polymerase chain reaction; R, reverse primer for plasmid construction.