Table 3.
Knockout/overexpression studies of Hippo pathway core components and impacts on endocrine function
| Study (first author, year) | Model system | Gene targeted | Overexpression/ knockout | Hormone measured | Findings |
|---|---|---|---|---|---|
| Fu et al., 2014 | KGN cell line | YAP1 | KO | E2 (conditioned media) | Reduces FSH-induced E2 production |
| Ye et al., 2017 | Lentiviral OE infertile mouse model | Yap1 | OE | E2, FSH (serum) | Increased FSH; no changes in E2 |
| Lentiviral KO wild-type mouse model | Yap1 | KO | E2, FSH (serum) | Decline in E2, FSH | |
| Lv et al., 2019 | KGN cell line 3D culture system | YAP1 | OE | E2, P4 (conditioned media) | Reduced E2, P4 |
| Plewes et al., 2019 | Primary bovine granulosa cells | YAP1 | KO | E2 (conditioned media) | Reduces FSH-induced E2 production |
| Lv et al., 2020 | KGN cell line | YAP1 | OE | E2, P4 (conditioned media) | Reduces FSH/FSK-induced E2, P4 production |
| Human granulosa cells from IVF patients | YAP1 | OE | E2, P4 (conditioned media) | Reduces FSH/FSK-induced E2, P4 production | |
| Lalonde-Larue et al., 2022 | Yap1 fl/fl ; Taz fl/fl ; Gnrhr GRIC mouse model | Yap1/Taz | KO | FSH, LH (serum) | Increases in LH; no changes in FSH |
| St. John et al., 1999 | Lats1 -/- mouse model | Lats1 | KO | LH, PRL, FSH (serum) | Deficiency in LH, PRL; no changes in FSH |
| Tsoi et al., 2019 | Lats1 fl/fl ; Lats2fl/fl; Cyp19a1-Cre mouse model | Lats1/2 | KO | E2, P4, LH, FSH (serum) | Decrease in P4; Increases in LH, FSH; E2 undetected (below assay threshold) |
Abbreviations: E2, estradiol; FSH, follicle-stimulating hormone; FSK, forskolin; KO, knockout; LH, luteinizing hormone; OE, overexpression; P4, progesterone; PRL, prolactin.