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. 2022 Nov 17;23(22):14209. doi: 10.3390/ijms232214209

Table 4.

RT-PCR setup.

Initial Denaturation 95 °C 3 min
PCR (35 cycles) Denaturation 95 °C 30 s
Annealing See Table 4 30 s
Extension 72 °C See Table 5
Final extension 72 °C 10 min