. 2022 Nov 17;23(22):14209. doi: 10.3390/ijms232214209

Table 5.

Primers used for RT-PCR.

Species	Gene	Direction	Primer Sequence	Product Size	Annealing Temp.	Extension Time	Positive Control
Mus musculus	Actb	Forward	GGCTGTATTCCCCTCCATCG	154 bp	55 °C	12 s	-
	Actb	Reverse	TTGAGCGAGGCTGCTGTTTC	154 bp	55 °C	12 s	-
	Tubb3	Forward	ATGAGGCCTCCTCTCACAAG	731 bp	56 °C	46 s	Brain
	Tubb3	Reverse	ATCGAACATCTGCTGCGTGA	731 bp	56 °C	46 s	Brain
	S1pr1	Forward	TTGAGCGAGGCTGCTGTTTC	466 bp	57 °C	30 s	Lung
	S1pr1	Reverse	CGCCTGCTAATAGGTCCGAG	466 bp	57 °C	30 s	Lung
	S1pr3	Forward	CTTCGGATTCTCTGGGGCAG	207 bp	56 °C	12 s	Lung
	S1pr3	Reverse	ATAGGCTCTCGTTCTGCAAGG	207 bp	56 °C	12 s	Lung
	S1pr4	Forward	AGCCAATGGGCAGAAGTCTC	773 bp	57 °C	46 s	Spleen
	S1pr4	Reverse	ACAGTAGCCTGGGCATTGAC	773 bp	57 °C	46 s	Spleen
	S1pr5	Forward	CCGGTTACAGGAGACTTTTGC	374 bp	55 °C	22 s	Brain
	S1pr5	Reverse	ACAGTAGGATGTTGGTGGCG	374 bp	55 °C	22 s	Brain
Rattus norvegicus	Actb	Forward	AGCCTTCCTTCCTGGGTATGG	361 bp	57 °C	22 s	-
	Actb	Reverse	GCAGCTCAGTAACAGTCCGC	361 bp	57 °C	22 s	-
	Gfap	Forward	AACCGCATCACCATTCCTGT	667 bp	57 °C	40 s	Brain
	Gfap	Reverse	TCTGCCCTACCCACTCCTAC	667 bp	57 °C	40 s	Brain
	S1pr1	Forward	TTGAGCGAGGCTGCTGTTTC	210 bp	57 °C	15 s	Lung
	S1pr1	Reverse	AGCCTTAACCACTGGGATGC	210 bp	57 °C	15 s	Lung
	S1pr3	Forward	GAACGAGAGCCTGTTTCCAAC	611 bp	56 °C	40 s	Spleen
	S1pr3	Reverse	TGCTTCTTGTTGGCGTCGTA	611 bp	56 °C	40 s	Spleen
	S1pr4	Forward	AACGGTTAGGCACAAGGAGG	248 bp	57 °C	15 s	Spleen
	S1pr4	Reverse	TTATGCTCAAGGTGCCCCAG	248 bp	57 °C	15 s	Spleen
	S1pr5	Forward	AGGCGCAAGGTTCGCATA	450 bp	56 °C	30 s	Brain
	S1pr5	Reverse	AGGAACATGGGTGCATGGAA	450 bp	56 °C	30 s	Brain