Table 1.
Biochemical characterization of B. pseudocatenulatum JCLA3.
| Strains | ||
|---|---|---|
| B. pseudocatenulatum JCLA3 | B. animalis subsp. lactis Bb-12 | |
| (a) Antibiotic profile * Vancomycin |
S | S |
| Ampicillin | R | R |
| Trimethoprim sulfamethoxazole | S | S |
| Gentamicin | S | S |
| Dicloxacillin | S | R |
| Cephalothin | R | R |
| Clindamycin | S | S |
| Erythromycin | S | S |
| Penicillin | S | S |
| Tetracycline | S | R |
| Cefotaxime | S | S |
| Ciprofloxacin | S | R |
| (b) Hemolysis | − | − |
| (c) Antimicrobial activity | ||
| E. coli ATCC 25922 | + | ++ |
| Escherichia coli O157: H7 | − | ++ |
| Salmonella typhi ATCC14028 | − | ++ |
| Staphylococcus aureus ATCC 6538 | + | + |
| (d) Bile salt tolerance assay | ||
| Glycocholic acid 0.1–0.5% | + | + |
| Taurocholic acid 0.1–0.5% | + | + |
| Glycodeoxycholic acid 0.1–0.5% | + | + |
| Taurodeoxycholic acid 0.1–0.5% | + | + |
| (e) Tolerance to GIT | ||
| Simulated saliva | 100% | 100% |
| Simulated gastric fluid | 0% | 2.26% |
| Simulated intestinal fluid | 0% | 0% |
* Antibiotic test: S: sensible; R: resistance; +: growth in the medium containing each bile salt. Antimicrobial activity: no inhibition (−), moderate (+), and total inhibition (++).