Table 6.
Mass spectrometry detection parameters (MRM mode).
| Analyte | RT/Min | Precursor Ion/m/z | Product Ion/m/z | Collision Energy/V |
|---|---|---|---|---|
| Goserelin | 4.61 | 635.50 | 607.45 | −20.0 |
| Buserelin | 4.88 | 620.45 | 248.90 | −37.0 |
| 592.35 | −19.0 | |||
| Triptorelin | 4.59 | 510.40 | 120.05 | −34.0 |
| Dalargin | 3.37 | 363.85 | 120.10 | −43.0 |
| 135.95 | −22.0 | |||
| 492.30 | −13.0 |
Total analysis time for a single injection was 10 min. The retention time for goserelin, buserelin, triptorelin, and dalargin (IS) was around 4.61 min, 4.88 min, 4.59 min, and 3.37 min, respectively.