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Sensitivity analysis. 1–10: 1.21 × 109–1.21 × 100 copies/μL (final reaction concentrations: 1.21 × 108 to 1.21 × 10−1 copies/μL) of the standard plasmids. The limit of detection (LOD) of all PEDV, TGEV, PDCoV, and SADS-CoV was 1.21 × 102 copies/μL (final reaction concentration: 1.21 × 101 copies/μL).
