Skip to main content
. 2022 Nov 16;9(11):634. doi: 10.3390/vetsci9110634

Table 1.

The sequences of the primers and probes used to differentiate PEDV, TGEV, PDCoV, and SADS-CoV.

Primer/Probe Sequence (5′→3′) Product/bp
PEDV(N)-U CTGGAATGAGCAAATTCGCTG 140
PEDV(N)-D CAACCCAGAAAACACCCTCAG
PEDV(N)-P JOE-AGCGAATTGAACAACCTTCCAATTGGCA-BHQ1
TGEV(M)-U GCAATTCTTTGCGTTAGTGCAT 102
TGEV(M)-D AGCGTACAAATTCCCTGAAAGC
TGEV(M)-P Texas Red-CTTCCTCTCGAAGGTGTGCCAACTGG-BHQ2
PDCoV(M)-U ATCGACCACATGGCTCCAA 72
PDCoV(M)-D CAGCTCTTGCCCATGTAGCTT
PDCoV(M)-P FAM-CACACCAGTCGTTAAGCATGGCAAGCT-BHQ1
SADS-CoV(N)-U TACTGGTCCTCACGCAGATG 120
SADS-CoV(N)-D ACGATTGCGAACACCAAGAC
SADS-CoV(N)-P Cy5-CAACAGCGACCCAATGCACACCCT-BHQ3