Table 2.
The components of the optimal reaction system.
| Reagent | Volume (µL) | Final Concentration (nM) |
|---|---|---|
| 2× One-Step RT-PCR Buffer III (TaKaRa) | 10 | / |
| Ex Taq HS (5 U/μL) (TaKaRa) | 0.4 | / |
| PrimeScript RT Enzyme Mix II (TaKaRa) | 0.4 | / |
| PEDV(N)-U (20 pmol/μL) | 0.2 | 200 |
| PEDV(N)-D (20 pmol/μL) | 0.2 | 200 |
| PEDV(N)-P (20 pmol/μL) | 0.3 | 300 |
| TGEV(M)-U (20 pmol/μL) | 0.3 | 300 |
| TGEV(M)-D (20 pmol/μL) | 0.3 | 300 |
| TGEV(M)-P (20 pmol/μL) | 0.3 | 300 |
| PDCoV(M)-U (20 pmol/μL) | 0.2 | 200 |
| PDCoV(M)-D (20 pmol/μL) | 0.2 | 200 |
| PDCoV(M)-P (20 pmol/μL) | 0.2 | 200 |
| SADS-CoV(N)-U (20 pmol/μL) | 0.3 | 300 |
| SADS-CoV(N)-D (20 pmol/μL) | 0.3 | 300 |
| SADS-CoV(N)-P (20 pmol/μL) | 0.3 | 300 |
| Nucleic acid template | 2.0 | / |
| RNase-free distilled H2O | Up to 20 | / |