Table 3.

The Ct values and coefficients of variation for repeatability analysis.

Plasmid	Concentration (Copies/μL)	Intra-Assay			Inter-Assay
		$\overline{\mathrm{x}}$	SD	CV (%)	$\overline{\mathrm{x}}$	SD	CV (%)
p-PEDV	1.21 × 109	11.96	0.13	1.09	11.88	0.04	0.34
	1.21 × 107	18.36	0.14	0.76	18.30	0.18	0.98
	1.21 × 105	24.44	0.06	0.25	24.52	0.16	0.65
p-TGEV	1.21 × 109	12.97	0.05	0.39	12.87	0.12	0.93
	1.21 × 107	19.26	0.03	0.16	19.12	0.17	0.89
	1.21 × 105	25.12	0.19	0.76	25.10	0.09	0.36
p-PDCoV	1.21 × 109	11.84	0.10	0.84	11.72	0.12	1.02
	1.21 × 107	18.27	0.27	1.48	18.55	0.10	0.54
	1.21 × 105	24.41	0.14	0.57	24.67	0.15	0.61
p-SADS-CoV	1.21 × 109	11.51	0.02	0.17	11.79	0.22	1.87
	1.21 × 107	17.81	0.06	0.34	17.95	0.18	1.00
	1.21 × 105	24.51	0.35	1.43	24.75	0.11	0.44