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Agreements of the detection results by the developed and the reference multiplex qRT-PCR.
| Method | Positive Samples | |||
|---|---|---|---|---|
| PEDV (%) | TGEV (%) | PDCoV (%) | SADS-CoV (%) | |
| The developed quadruplex qRT-PCR | 591/3236 (18.26%) | 15/3236 (0.46%) | 426/3236 (13.16%) | 5/3236 (0.15%) |
| The reference multiplex qRT-PCR | 572/3236 (17.67%) | 15/3236 (0.46%) | 417/3236 (12.89%) | 5/3236 (0.15%) |
| Agreements | 99.41% | 100% | 99.72% | 100% |
