Figure 2.

Percent inhibition of HIV-1 replication in TZM-bl cells by ellagic acid (EA). EA was added either 1 h before (“pre-treatment”) or after 4 h of exposure to HIV-1_{JRCSF/NanoLuc} and following washing off the virus (“post-treatment”). HIV replication in the cells was measured by detection of nano luciferase secreted into the cell culture supernatants using the Nano-Glo Luciferase detection assay 48 h post-challenge and was compared to HIV-1 infection without EA to calculate %Inhibition. %Inhibition = [(replication without EA−replication with EA)/replication without EA] × 100. Bars show the mean + SD of three independent experiments. All differences were significant at p-values of <0.05 versus the untreated control.