Figure 3.

Percent inhibition of HIV-1 replication in U87CD4.CCR5 cells by ellagic acid (EA) showed a dose-dependent result. Cells were incubated for 24 h, were treated with the indicated concentrations of EA, and were incubated again for an additional hour. Then, the cells were infected with the HIV-1_{JRCSF/NanoLuc} virus and were incubated for 48 h. HIV replication in the cells was measured by detection of nano luciferase secreted into the cell culture supernatants using the Nano-Glo Luciferase detection assay 48 h post-challenge, and the treated conditions were compared to HIV-1 infection without EA to calculate %Inhibition. %Inhibition = [(replication without EA−replication with EA)/replication without EA] × 100.