Figure 6.

Percent inhibition of in vitro HIV integrase activity by Ellagel. Two different formulations of Ellagel, containing either 100 μM or 200 μM ellagic acid (EA), were tested. Effects of Ellagel on HIV-1 integrase activity were determined using an in vitro integration assay, in which digoxigenin-labelled target DNA was incubated with HIV-1 integrase and plate-bound HIV-long-terminal repeat (LTR) DNA in the presence or absence of Ellagel. After incubation and washing, DNA integration was detected by an alkaline phosphatase-labeled anti-digoxigenin antibody. The optical density (OD) of the AP-labeled antibody was measured at 405 nm. Anti-HIV-1 integrase activity was calculated as follows: % Anti-HIV-1 integrase activity = [(OD control − OD sample)/(OD control)] × 100. Mean % inhibition ± standard deviation of four replicates is shown in the plot.