. 2022 Nov 11;27(22):7773. doi: 10.3390/molecules27227773

Table 1.

Qualitative–quantitative analysis of phenolic compounds in buckwheat forms.

Compound		Rt	λ_max	(M-H) m/z		Sample
Compound		min	nm	MS	MS/MS	Seeds (G)	Control Sprouts (CS)	Probiotic-Rich Sprouts (PRS)
Phenolic acids
1	Caffeoyl-glucoside	2.19	288	341	179. 143	47.29 ± 0.41 ^a	53.23 ± 0.06 ^a,b	55.48 ± 0.52 ^b
2	Caffeoyl-rhamnopyranosyl-glucopyranosyl-glucopyranoside	2.47	289	649	487. 179	24.07 ± 0.54 ^a	27.80 ± 0.25 ^a	29.57 ± 0.92 ^a
3	Caffeoyl-rhamnopyranosyl-glucopyranosyl	3.50	288	487	179	79.80 ± 0.30 ^a	92.04 ± 0.05 ^b	118.23 ± 0.04 ^c
Flavan-3-ols
1	Unknown catechin derivate	2.57	272	535	515. 267	73.52 ± 0.01 ^c	61.92 ± 0.23 ^b	61.89 ± 0.08 ^a
2	(Epi) afzelechin-(epi)-catechin	2.76	277	561	289. 245	9.47 ± 0.25 ^a	38.24 ± 0.89 ^b	56.80 ± 0.13 ^c
3	Catechin-glucoside	2.91	276	451	289	39.06 ± 0.43 ^a	62.08 ± 0.94 ^b	113.90 ± 0.71 ^c
4	Catechin-3-O-glucoside-6-O-rutinoside	3.09	278	719	451. 289	29.55 ± 0.07 ^a	32.60 ± 0.29 ^b	46.19 ± 0.92 ^c
5	Caffeoyl-glucoside	3.31	288	341	179	2.31 ± 0.99 ^a	17.55 ± 0.5 ^b	33.51 ± 0.38 ^c
6	(+)Catechin	3.34	276	289	-	11.59 ± 0.11 ^a	27.85 ± 0.1 ^b	45.14 ± 0.75 ^c
7	Catechin-glucoside	3.40	279	451	289	18.35 ± 0.11 ^a	29.78 ± 0.2 ^b	42.66 ± 0.21 ^c
8	Epicatechin-(4-8)-epicatechin	3.61	277	577	289	107.36 ± 0.19 ^a	100.88 ± 0.05 ^a	115.94 ± 0.62 ^b
9	Epicatechin-(4-8)-epigallocatechin-gallate	3.70	279	729	577. 407. 289	15.79 ± 0.54 ^a	34.96 ± 0.76 ^b	57.89 ± 0.40 ^c
10	Epicatechin gallate dimethyl derivative	3.81	284	469	425. 137	3.45 ± 0.42 ^a	49.86 ± 0.48 ^b	63.37 ± 0.93 ^c
11	Epicatechin gallate	3.97	317	883 2 [M-H]⁻	441. 289	27.17 ± 0.805 ^a	28.55 ± 0.45 ^a	70.88 ± 0.34 ^b
12	(-)Epicatechin	4.14	277	289	-	14.01 ± 0.74 ^a	39.60 ± 0.62 ^b	66.55 ± 0.07 ^c
13	Catechin trimer	4.27	279	865	577. 289	11.26 ± 0.01 ^a	19.80 ± 0.32 ^b	32.73 ± 0.71 ^c
14	Epicatechin gallate methyl derivative	4.44	292	455	441. 289	7.20 ± 0.86 ^a	113.27 ± 0.02 ^b	138.25 ± 0.98 ^c
15	Epicatechin trimer	4.62	279	865	577. 289	60.67 ± 0.18 ^b	22.61 ± 0.11 ^a	57.14 ± 0.3 ^b
16	Epiafzelechin-epicatechin-gallate dimethyl derivative	6.92	279	741	605. 469. 271	20.29 ± 0.22 ^a	10.75 ± 0.35 ^a	19.21 ± 0.98 ^a
17	Epiafzelechin-epicatechin-gallate methyl derivative	7.88	271	727	601. 407. 289	21.39 ± 0.2 ^b	5.68 ± 0.16 ^a	12.32 ± 0.62 ^a
Flavonols
1	Orientin	4.67	269. 347	447	285	7.31 ± 0.43 ^a	3.44 ± 0.54 ^a	43.51 ± 0.42 ^b
2	Isorientin	4.87	270. 312	447	285	ND	6.42 ± 0.56 ^a	35.83 ± 0.25 ^b
3	Quercetin-3-O-rutinoside	5.24	255. 352	609	301	41.28 ± 0.88 ^a	52.48 ± 0.34 ^b	88.37 ± 0.42 ^c
4	Vitexin	5.35	269. 329	431	269	ND	20.09 ± 0.87 ^a	121.03 ± 0.58 ^b
Total phenols compounds (µg/g d.w.)						672.14 ± 0.92 ^a	951.42 ± 1.82 ^b	1526.34 ± 3.33 ^c

Mean values with different letters in the row are statistically significantly different, with significance indicated as p ≤ 0.05. “±” means standard deviation. ND—not detected.