Figure 2.

Uptake of 50 nM [³H]-adenosine by LmexNT1.1 expressed in L. mexicana promastigotes from which both original alleles of LmexNT1.1/NT1.2 and of LmexNT2 had been deleted by CRISPR/cas9. Incubation time was 10 s, well within the linear phase of uptake [30]. Symbols represent averages of triplicate determinations, and error bars indicate ± SEM when not shown error bars fall within the symbol. The graph shown is a single experiment in triplicate, representative of three identical, independent repeats.