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. 2022 Nov 21;27(22):8087. doi: 10.3390/molecules27228087

Table 5.

Effects of tubercidin and its analogues on promastigotes of the LmexCas9, LmexΔNT1 and LmexΔNT1+TcrNT2 strains.

Cas9 Cas9ΔNT1 LmexΔNT1+TcrNT2
EC50 (μM) EC50 (μM) EC50 (μM) Sensitization (fold) p vs. Cas9ΔNT1
Tubercidin 1.02 ± 0.16 25.7 ± 0.94 0.82 ± 0.13 31.2 <0.001 graphic file with name molecules-27-08087-i001.jpg
1 (FH11706) >100 >100 ND graphic file with name molecules-27-08087-i002.jpg
2 (FH10714) >100 >100 >100 graphic file with name molecules-27-08087-i003.jpg
TH1012 52.5 ± 0.3 280 ± 18 96.6 ± 0.3 2.9 <0.001 graphic file with name molecules-27-08087-i004.jpg
FH3147 8.41 ± 1.61 >100 13.7 ± 1.25 7.3 <0.001 graphic file with name molecules-27-08087-i005.jpg
FH3169 0.24 ± 0.06 2.00 ± 0.19 0.32 ± 0.05 6.3 <0.01 graphic file with name molecules-27-08087-i006.jpg
TH1004 37.0 ± 0.23 138 ± 10 101 ± 0.5 1.37 <0.05 graphic file with name molecules-27-08087-i007.jpg
3 (FH8513) >100 100.5 ± 0.9 31.1 ± 0.5 3.2 <0.01 graphic file with name molecules-27-08087-i008.jpg
Pentamidine 0.87 ± 0.10 1.25 ± 0.22 1.12 ± 0.17 1.11 >0.05

EC50, 50% effective concentration determined in a resazurin-based assay; sensitization is determined as the ration of the EC50 values of the Cas9ΔNT1 and Cas9ΔNT1+TcrNT2 strains. The p value is based on an unpaired, two-tailed Student’s t-test comparing the EC50 values of the same two strains (n ≥ 3).