Table 1.
List of tentatively identified compounds from Skeletonema grevillei extracts by GC-MS in bioreactor after 192 and 312 h.
| No. | Retention Index | Similarty (%) | Proportion (%) | Identified Compound | Molar Weight | |||
|---|---|---|---|---|---|---|---|---|
| BRC192 | BRC312 | BRC192 | BRC312 | BRC192 | BRC312 | |||
| 1 | 1242 | n.d. | 96 | n.d. | 0.05 | n.d. | Benzoic acid, TMS derivative | 194 |
| 2 | 1253 | n.d. | 86 | n.d. | 0.02 | n.d. | Octanoic acid, TMS derivative | 216 |
| 3 | 1269 | 1265 | 94 | 92 | 0.13 | 0.21 | Glycerol, 3TMS derivative | 308 |
| 4 | 1348 | 1345 | 95 | 92 | 0.08 | 0.05 | Nonanoic acid, TMS derivative | 230 |
| 5 | 1451 | 1450 | 95 | 91 | 0.02 | 0.02 | Decanoic acid, TMS derivative | 244 |
| 6 | n.d. | 1499 | n.d. | 94 | n.d. | 0.13 | Malic acid, 3TMS derivative | 350 |
| 7 | 1534 | n.d. | 91 | n.d. | 0.01 | n.d. | 1-Dodecanethiol | 202 |
| 8 | 1653 | 1499 | 97 | 93 | 0.05 | 0.03 | Dodecanoic acid, TMS derivative | 272 |
| 9 | 1691 | n.d. | 85 | n.d. | 0.03 | n.d. | Tetradecanenitrile | 326 |
| 10 | 1753 | 1752 | 94 | 94 | 0.10 | 0.14 | Tridecanoic acid, TMS derivative | 286 |
| 11 | 1767 | n.d. | 91 | n.d. | 0.01 | n.d. | 1-Tetradecanol, TMS derivative | 286 |
| 12 | 1788 | 1787 | 93 | 93 | 0.06 | 0.17 | Loliolide, TMS | 268 |
| 13 | 1853 | 1852 | 95 | 96 | 2.55 | 6.50 | Myristic acid, TMS derivative | 300 |
| 14 | n.d. | 1877 | n.d. | 90 | n.d. | 0.28 | (Z)-3-Hexenyl β -glucopyranoside, 4TMS derivative | 550 |
| 15 | 1879 | n.d. | 94 | n.d. | 0.11 | n.d. | Palmitoleonitrile | 235 |
| 16 | 1930 | n.d. | 86 | n.d. | 0.05 | n.d. | (E)-13-Methyltetradec-9-enoic acid, TMS derivative | 312 |
| 17 | 1952 | 1951 | 94 | 85 | 0.17 | 0.49 | Pentadecanoic acid, TMS derivative | 314 |
| 18 | 2009 | 2008 | 89 | 88 | 0.97 | 0.63 | Eicosapentaenoic acid, TMS derivative | 374 |
| 19 | 2031 | 2030 | 96 | 97 | 7.37 | 12.15 | Palmitelaidic acid, TMS | 326 |
| 20 | 2053 | 2052 | 96 | 95 | 2.31 | 2.73 | Palmitic acid, TMS derivative | 328 |
| 21 | 2076 | n.d. | 89 | n.d. | 0.43 | n.d. | 9-Octadecynenitrile | 261 |
| 22 | 2083 | 2082 | 97 | 97 | 2.96 | 0.38 | (Z)-9-Octadecenenitrile | 263 |
| 23 | 2151 | 2094 | 92 | 83 | 0.04 | 0.03 | Heptadecanoic acid, TMS derivative | 342 |
| 24 | n.d. | 2157 | n.d. | 93 | n.d. | 0.04 | Palmitoleamide | 253 |
| 25 | n.d. | 2163 | n.d. | 87 | n.d. | 0.01 | 1-Octadecanol, TMS derivative | 342 |
| 26 | n.d. | 2178 | n.d. | 85 | n.d. | 0.03 | Octadecanamide | 319 |
| 27 | 2184 | 2184 | 92 | 97 | 1.48 | 2.09 | Phytol, TMS derivative | 368 |
| 28 | 2216 | 2215 | 88 | 92 | 0.47 | 0.44 | Linoleic acid, TMS derivate | 352 |
| 29 | 2230 | 2229 | 94 | 94 | 2.36 | 0.90 | (Z)-Oleic acid, TMS derivative | 354 |
| 30 | 2251 | 2250 | 97 | 95 | 0.88 | 0.65 | Stearic acid, TMS derivative | 356 |
| 31 | n.d. | 2256 | n.d. | 86 | n.d. | 0.05 | 11-Methyloctadec-12-enoic acid, TMS derivative | 368 |
| 32 | n.d. | 2331 | n.d. | 90 | n.d. | 0.16 | (all-Z)-5,8,11,14,17-Eicosapentaenoic acid, methyl ester | 330 |
| 33 | 2337 | n.d. | 92 | n.d. | 0.10 | n.d. | (Z)-10-Nonadecenoic acid, TMS | 368 |
| 34 | 2365 | 2362 | 95 | 96 | 2.30 | 2.50 | 9-Octadecenamide | 281 |
| 35 | n.d. | 2373 | n.d. | 90 | n.d. | 0.68 | (Z)-5,8,11-Eicosatrienoic acid, TMS derivative | 378 |
| 36 | 2381 | 2380 | 94 | 96 | 3.29 | 6.00 | Eicosapentaenoic acid, TMS derivative | 374 |
| 37 | 2430 | 2422 | 83 | 82 | 49.84 | 33.65 | Oleamide, TMS derivative | 353 |
| 38 | 2450 | 2448 | 95 | 95 | 1.24 | 0.70 | Steramide, TMS derivative | 355 |
| 39 | 2564 | 2564 | 97 | 89 | 0.54 | 0.19 | Doconexent, TMS derivative | 400 |
| 40 | 2578 | 2578 | 93 | 87 | 1.39 | 1.09 | 2-Palmitoylglycerol, 2TMS derivative | 474 |
| 41 | 2610 | 2610 | 96 | 96 | 2.91 | 2.89 | 1-Monopalmitin, 2TMS derivative | 474 |
| 42 | 2771 | 2770 | 90 | 90 | 1.46 | 1.59 | 2-Monostearin, 2TMS derivative | 502 |
| 43 | 2787 | 2787 | 93 | 90 | 0.34 | 0.10 | 1-Monooleoylglycerol, 2TMS derivative | 500 |
| 44 | 2805 | 2804 | 97 | 96 | 5.92 | 9.30 | Glycerol monostearate, 2TMS derivative | 502 |
| 45 | 2826 | 2825 | 88 | 81 | 0.57 | 0.10 | (Z)-Docos-13-enamide, TMS | 409 |
| 46 | n.d. | 2997 | n.d. | 89 | n.d. | 0.15 | Eicosanoic acid, 2,3-bis-(OTMS) propyl ester | 530 |
| 47 | 2998 | n.d. | 93 | n.d. | 0.09 | n.d. | 2,3-Dihydroxypropyl icosanoate, 2TMS derivative | 530 |
| 48 | 3066 | 3066 | 89 | 88 | 0.09 | 0.10 | 2-Arachidonoylglycerol, 2TMS derivative | 522 |
| 49 | 3163 | 3162 | 96 | 96 | 3.97 | 6.21 | Cholesterol, TMS derivative | 458 |
| 50 | n.d. | 3201 | n.d. | 90 | n.d. | 0.40 | Desmosterol, TMS derivative | 456 |
| 51 | 3261 | 3260 | 90 * | 88 * | 3.03 | 5.09 | 24-Methylene cholesterol * | 398 * |
| 52 | 3380 | 3380 | 88 | 81 | 0.15 | 0.20 | Isofucosterol, TMS | 484 |
| 53 | 3672 | 3672 | 90 | 92 | 0.06 | 0.80 | Oleanolic acid 2TMS | 600 |
* A compound identified in non-derivatized form and its molecular weight (other data for this compound, RI, and proportion, were obtained for the derivatized form) as well as with literature data comparison for its derivatized form.; n.d.—not detected. BRC192—after 192 h in a bioreactor; BRC312—after 312 h in a bioreactor.