FIG. 1.
Comparison of flagellins isolated from Campylobacter spp. and MBP-FlaA isolated from E. coli. Proteins were separated by SDS-PAGE in an 8.25% gel and either stained with Coomassie blue (A) or immunodetected with various antisera (B to E): B, E288 rabbit antiserum which was generated against denatured strain VC167 T2 flagellin (37) and cross-absorbed with whole cells of E. coli DH5α at a final dilution of 1:4,000 (37); C, LL1 antiserum against MBP-FlaA at a dilution of 1:20,000; D, ferret antiserum from an animal infected with strain 81-176 at a dilution of 1:500; E, human antiserum from a human volunteer infected with 81-176 at a dilution of 1:5,000. Lane 1, VC167 T2 flagellin; lane 2, 81-176 flagellin; lane 3, MBP-FlaA. VC167 T2 flagellin migrates at an apparent Mr of 59,500 (16, 18), and 81-176 flagellin migrates at an apparent Mr of 62,000 (39).