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. 2022 Nov 3;9(11):379. doi: 10.3390/jcdd9110379

Figure 2.

Figure 2

In-house-developed optical setup for performing SS-OCT in a fiber based, Mach-Zehnder configuration. The swept source laser is connected to the interferometer via a 90/10 fiber coupler (FC) transmitting 10% of the optical power to the reference arm via a circulator (C). The collimator (Coll) collimates the laser light, and a lens (L), filter wheel (F), and mirror (M) are mounted on a linear translation stage to match the arm lengths. 90% of the optical power is transmitted to the sample arm in which scanning is performed with galvanometric scanners (GS). The scan lens (SL) focuses the laser light onto the embryo, which is mounted on a three-axis translation stage. Reference and sample arm signals are combined in a 50/50 fiber coupler and detected in a dual balanced detector (DBD). The DBD is connected to the computer for data acquisition as well as driving the swept source laser and the GS. The polarization paddles (PP) provide adjustment capabilities for the polarization state of the laser light.