Virtual thick section of a micro-CT scan of a PTA stained HH16 chick embryo heart in sagittal plane showing the OFT and ventricle at early onset of cells migrating into the endocardial cushions (EndMT) and the two-layered myocardium in near histological resolution. The cardiac jelly (CJ) appears empty as the collagens, glycoproteins, and mucopolysaccharides that it consists of do not stain sufficiently with PTA to be detectable at this resolution. Note that blood cells stain heavily with PTA. bl-c blood cells, ce cell nuclei, CJ cardiac jelly, endo endocardium, lu lumen, myo myocardium, te tether. scale bar = 500 µm.