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. 1999 Nov;67(11):5815–5819. doi: 10.1128/iai.67.11.5815-5819.1999

FIG. 1.

FIG. 1

PCR analysis of mutants. Chromosomal DNA from strain 7169 and the isogenic mutants 7169b12 (TbpA+ TbpB−), 7169tbpA35 (TbpA− TbpB+), and 7169tbpAB-1 (TbpA− TbpB−) was subjected to PCR analysis, and the products were separated by electrophoresis. Products in lanes A, C, E, and G were obtained with tbpB-specific primers 42 and 43 (described in Materials and Methods). Lanes B, D, F, and H were obtained with primers 61 and 63, which are specific for sites within tbpA. Both primer sets flank the predicted site of the aphA-3 cassette insertion within the mutants. Lanes: A and B, strain 7169; C and D, 7169b12; E and F, 7169tbpA35; G and H, 7169tbpAB-1. A negative image of an ethidium bromide-stained agarose gel is shown with molecular size standards, in kilobases, to the left.