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Growth of parental strain 7169 and the isogenic mutants with Tf as the sole source of iron. Iron-depleted overnight cultures of each strain were used to inoculate fresh CDM 0 or CDM containing 5 μM human holotransferrin. All cultures contained 10 μM desferoxamine. The optical density at 600 nm (OD600) of each culture was measured in 2-h intervals for 10 h. Symbols: ●, 7169-Tf; ⧫, 7169b12-Tf; ■, 7169tbpA35-Tf; ▴, 7169tbpAB1-Tf; ○, 7169-0.
