Figure 4.

Differential cytotoxicity due to exposure of flavored oral smokeless nicotine products in bronchial epithelial cells. (A) BEAS-2B cells were seeded and treated with spearmint-flavored oral product extracts from two different brands. Following 24 h exposure, conditioned media was used for the lactate dehydrogenase (LDH) measurements. (B) 16HBE cells were seeded and treated with ON! Original, Rogue mango, or Velo black cherry-flavored nicotine pouch extracts. Following 24 h exposure, conditioned media was used LDH measurements. Data represented as means *** p < 0.001 compared to control. (Mean ± SEM, n = 3.)