Table 1.

Primer design for Mus musculus.

Gene Symbol	Forward Primer	Reverse Primer	Probe (Roche)	Accession Number
Housekeeping
36B4	F: 5′-AAGCGCGTCCTGGCATTGTCT-3′	R: 5′-CCGCAGGGGCAGCAGTGGT-3′	72	NM_007475.4
Genes of interest
GPR21	F: 5′-GAACTCCACCTGGGATGGTA-3′	R: 5′-GTAGCCCAGTGCCAGAAGAC-3′	46	NM_177383.4
GPR26	F: 5′-GCCAGAGCAAAGGGAGGT-3′	R: 5′-AGGCAATGGTGGCAGTTATT-3′	10	NM_173410.3
GPR39	F: 5′-CGGCGCAGTAACTCTTCC-3′	R: 5′ -GGCCTCAGTCTGAAAAGTGC-3′	74	NM_027677.2
GPR82	F: 5′-GGAACAGAAAATATGACCTGATTCAT-3′	R: 5′- GAGGGCCTAGCACATAGCAA ′	36	NM_175669.4
GPR6	F: 5′-ACATGCCAGCCTTTGGTG-3′	R: 5′-GCACCACTGACACCTCAAGA-3′	40	NM_199058.2
TNF- α	F: 5′-TCTTCTCATTCCTGCTTGTGG-3′	R: 5′-GGTCTGGGCCATAGAACTGA -3′	49	NM_001278601.1
IL-6	F: 5′-ACAAAGCCAGAGTCCTTCAGA-3′	R: 5′-TGGTCCTTAGCCACTCCTTC-3′	78	NM_001314054.1
AdipoQ	F: 5′-GGCTCTGTGCTCCTCCATCT-3′	R: 5′-AGAGTCGTTGACGTTATCTGCA-3′	1	NM_009605.5
IL-10	F: 5′-CCCTGGGTGAGAAGCTGAAG-3′	R: 5′-GGGGAAGAACGCATCTGCTA-3′	30	NM_010548.2