TAK-242 treatment inhibited the proliferation, and changed the expression, of EMT related proteins induced by PA in the B16 and A375 cells. (A) The level of cell proliferation after B16 and A375 cells were incubated with TAK-242. (B) The level of cell proliferation after B16 and A375 cells were incubated with TAK-242 and/or PA for 24 h. (C) Protein levels and quantitative analysis of E-cadherin, MMP2, and Vimentin in B16 cells incubated with TAK-242 and/or PA for 24 h. (D) Protein levels and quantitative analysis of E-cadherin, MMP2, and Vimentin in A375 cells incubated with TAK-242 and/or PA for 24h. In A375 and B16 cells, treatment with PA or TAK-242 (+) and no treatment with PA or TAK-242 (-). * p < 0.05, ** p < 0.01, or *** p < 0.001 compared with the control group. ## p < 0.01, or ### p < 0.001 compared with the PA group; the significance of the ANOVA.