The fluorescence intensity and protein expression of TLR4/TRIF-Peli1-NF-κB pathway after si-Peli1. (A) The fluorescence intensity of TLR4 in A375 cells. (B) The fluorescence intensity of TRIF in A375 cells. (C) The fluorescence intensity of Peli1 and pNF-κB in A375 cells. (D) Quantitative analysis of fluorescence intensity. (E) The protein blots and quantitative analysis of TLR4, TRIF, and pNF-κB in B16 cells. In A375 and B16 cells, treatment with PA or si-Peli1 (+) and no treatment with PA or si-Peli1 (-). * p < 0.05, ** p < 0.01, or *** p < 0.001 compared with the control group. ## p < 0.01 or ### p < 0.001 compared with the PA group; the significance of the ANOVA.