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Uptake of usGNPs depends on αVβ3 integrin expression level. (a–d) U-87 MG and U-251 MG cells were incubated with 9 nM usGNPs with indicated cRGD densities for 2 h, then stained with 1 µg/mL Hoechst 33342 and imaged with live cell confocal microscopy. (e) Internalized usGNP content was quantified with ICP-AES following 2 h incubation. Representative figure from three (U87-MG) and one (U251-MG) independent repeats; error bars represent SD of five technical replicates.
