FIG. 4.

Effects of LPS on LRP mRNA expression in HS-P cells. HS-P cells were treated with 100 ng of LPS per ml for the indicated times. (A) Northern blot analysis of total RNA (20 μg per lane) was performed with a rat LRP (rLRP) cDNA probe. The lower panel shows the results after hybridizing the blot with a 7S RNA cDNA probe, which was used as a load control. The results shown are from a representative Northern blot repeated three times. (B) Relative intensity of LRP at 0, 0.5, 1, 2, 4, and 6 h following LPS exposure normalized to 7S and expressed as a percentage of time zero. The results shown are means ± standard errors of three separate experiments. LRP was significantly decreased at all time points (P < 0.05).