FIG. 5.

Effect of LPS on HS-P α₂M* internalization. HS-P cells were treated with 100 ng of LPS per ml for 24 h, washed, and incubated at 37°C for 2 h with ¹²⁵I-α₂M* (4 nM), in the presence or absence of excess unlabeled ligand. Cells were washed, collected, and lysed, and radioactivity was determined. The results shown are means ± standard errors of triplicate samples from three separate experiments (n = 9). Internalization was significantly decreased after LPS treatment (P < 0.05).