. 2022 Nov 7;11(11):1305. doi: 10.3390/pathogens11111305

Table 2.

Target genes, primer sequences, amplicon sizes, cycling conditions and references of PCRs.

Target Gene	Primers Sequences	Amplified Segment (bp *)	Primary Denaturation (94 °C)	Amplification (35 Cycles)			Final Extension (72 °C)	Reference
Target Gene	Primers Sequences	Amplified Segment (bp *)	Primary Denaturation (94 °C)	Secondary Denaturation (94 °C)	Annealing	Extension (72 °C)	Final Extension (72 °C)	Reference
Toxoplasma gondii B1	5′-GGAACTGCATCCGTTCATGAG-3′	196	5 min	30 s	60 °C 30 s	30 s	7 min	[41]
Toxoplasma gondii B1	5′-TCT TTA AAG CGT TCG TGG TC-3′	196	5 min	30 s	60 °C 30 s	30 s	7 min	[41]
Neospora caninum Nc5	5′-CCCAGTGCGTCCAATCCTGTAAC-3′	337	5 min	30 s	63 °C 40 s	40 s	10 min	[42]
Neospora caninum Nc5	5′-CTCGCCAGTCAACCTACGTCTTCT-3′	337	5 min	30 s	63 °C 40 s	40 s	10 min	[42]

* bp; base pairs.