Table 3.

Analysis Method of paper-based microfluidic chips.

Methods	Advantages	Disadvantages
Colorimetric method	Intuitive results, easy to read with the naked eye, low cost [51,52]	Unable to achieve accurate quantitative detection [53]
Electrochemical method	Quantitatively accurate, fast reading [54,55]	Rely on electrochemical workstation, increase cost and reduce flexibility [56]
Fluorescence method	Low detection limit, very sensitive [57,58]	Relying on fluorescence detection equipment, easily affected by the signal of paper fluorescent agent [59]
United electronics	Combining the aforementioned methods enables non-professionals to obtain accurate results [60,61]	Need to install a mobile APP or even larger devices, reducing flexibility [4]