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S. flexneri 2a O-antigen-specific ASC responses detected 7 days after the second immunization in the SVCLN (A) and spleen (B) and the total ASC response (C) from animals immunized intranasally with EcSf2a-3 with and without toxin molecules LT and mLT(R192G). Each data point represents the mean and standard error of the mean of values from eight animals. MNC, mononuclear cells; NC, nonimmunized control animals.
