Figure 4.

Analysis of ROS contents and measurement of physiological and biochemical parameters. (A) The leaves of WT, ls1-1, and ls1-2 plants with no treatment at the heading stage. (B–D) NBT-, TB-, and DAB-stained leaves of WT and ls1 plants. (E,F) Measurement of the H₂O₂ and MDA contents in WT and ls1 plants. (G,H) Activities of the ROS-related enzymes SOD and CAT in WT and ls1 plants. The error bars indicate SDs of three biological replicates. The p-value was calculated using a Student’s t-test. ** p < 0.01.