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Programmed cell death (PCD) detection in the leaves of ls1 plants. The red signal represents staining with propidium iodide (PI); yellow and green signals indicate TUNEL-positive nuclei of dead cells due to PCD. WT and ls1 leaves were analyzed at the heading stage. The green fluorescence (520 nm) of apoptotic cells (TUNEL) in a red (620 nm) background (propidium iodide, PI) was detected with a Zeiss LSM880 confocal laser scanning microscope. Scale bar = 100 μm.
