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. Author manuscript; available in PMC: 2023 Apr 6.
Published in final edited form as: Nat Struct Mol Biol. 2022 Oct 6;29(10):990–999. doi: 10.1038/s41594-022-00838-z

Extended Data Fig. 5. Raw NMR spectra for wild-type SMO peptide binding to PKA-Cα, as shown in Fig. 2a, and PKIα(5–24)-induced displacement of SMO peptide from PKA-Cα, as shown in Fig. 2c.

Extended Data Fig. 5

a, 2D NMR spectra for ATPγN-bound PKA-Cα, either alone (left) or with SMO peptide added at a 1:6 kinase:peptide ratio (right). b, 2D NMR spectra for titration of PKIα(5–24) peptide into the SMO peptide:ATPγN:PKA-Cα complex at the indicated kinase: PKIα(5–24) ratios. Upper left panel represents an overlay of the individual spectra to highlight the concentration dependence of the PKIα(5–24) peptide-induced effects.