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. Author manuscript; available in PMC: 2023 Apr 6.
Published in final edited form as: Nat Struct Mol Biol. 2022 Oct 6;29(10):990–999. doi: 10.1038/s41594-022-00838-z

Fig. 3: SMO inhibits PKA-C enzymatic activity.

Fig. 3:

a, Spectrophotometric assay of PKA-Cɑ substrate phosphorylation, in the presence of standard (red) or extended (coral) SMO peptides (see Fig. 1a) or a control PKIα(5–24) peptide (grey). b, Concentration dependent inhibition of PKA-Cɑ with recombinant SMO pCT. c, As a, but comparing wild-type vs. WRR mutant versions of the recombinant SMO pCT.Inhibition in a-c is calculated relative to a control without SMO or PKIα(5–24) peptide (Ctrl.). Data in a-c is shown as the mean +/− standard deviation of two independent experiments. P < 0.001 (***); P < 0.0001 (****), n.s. = not significant. See Supplementary Table 1 for full statistical analysis.