Skip to main content
. 2022 Nov 21;27(22):8111. doi: 10.3390/molecules27228111

Figure 3.

Figure 3

Effects of TBBPA on oxidative stress and apoptosis in oocytes: (A) Generation of ROS after TBBPA treatment. Ex/Em, 488/530 nm. Scale bar = 100 μm. (B) ROS levels were reflected by the mean fluorescent intensity of ROS. Control, n = 79; TBBPA, n = 81. (C) Representative images of GSH after TBBPA treatment. Ex/Em, 371/464 nm. Scale bar = 100 μm. (D) Mean fluorescent intensity of GSH. Control, n = 108; TBBPA, n = 114. (E) Representative images of DHE after TBBPA treatment. Ex/Em, 529/590. Scale bar = 100 μm. (F) Mean fluorescent intensity of DHE. Control, n = 74; TBBPA, n = 83. (G) Protein expression level of SOD2 after TBBPA treatment. Ex/Em, 488/530 nm. Scale bar = 100 μm. (H) Mean fluorescent intensity of SOD2. Control, n = 58; TBBPA, n = 56. (I) Annexin-V levels after TBBPA treatment. Ex/Em, 488/530 nm. Scale bar = 20 μm. (J) Apoptosis rate in each group. ** p < 0.01, *** p < 0.001.