Skip to main content
. 2022 Oct 22;10(11):2094. doi: 10.3390/microorganisms10112094

Table 2.

Antibacterial activity of Lp. plantarum strains against Ps. syringae pv. syringae by agar diffusion assay.

LAB Strain Treatment
CFS Trypsin pH Proteinase K Catalase Boiling
Inhibition Zone, mm
C6 16.00 ± 1.02 d** 3.10 ± 0.79 c 1.17 ± 0.36 b 0.00 a 0.00 a 3.03 ± 0.33 c**
D16 23.67 ± 0.93 d** 0.00 a 0.00 a 0.00 a 18.00 ± 1.36 d** 0.00 a
I6 16.00 ± 0.85 d** 0.00 a 2.03 ± 0.26 d** 1.67 ± 0.59 b 0.00 a 0.00 a
L1 12.17 ± 0.76 c 26.67 ± 0.92 e** 0.00 a 0.00 a 1.33 ± 0.23 b 0.00 a
K1 2.33 ± 0.65 a 0.00 a 1.63 ± 0.37 c 0.00 a 0.00 a 0.00 a
Q3 15.67 ± 0.83 d** 5.33 ± 0.46 d** 1.77 ± 0.59 cd** 2.50 ± 0.74 c** 0.00 a 0.00 a
Q4 23.67 ± 0.98 e** 3.17 ± 0.85 c 2.53 ± 0.83 e** 0.00 a 2.33 ± 0.47 c** 1.90 ± 0.42 b**
S1 5.80 ± 0.57 b 2.00 ± 0.45 b 2.00 ± 0.72 d** 0.00 a 2.23 ± 0.49 c** 0.00 a
S4 1.70 ± 0.32 a 0.00 a 0.00 a 1.330 ± 0.46 b 1.70 ± 0.39 bc 0.00 a

Note: CFS—cell-free supernatant, Trypsin—CFS + trypsin of 1 mg/mL, pH—pH adjusted from 3.5–4.2 to 6.5, Proteinase K—CFS + proteinase K (100 U/mg), Catalase—CFS + catalase (5 mg), Boiling—boiling of CFS for 20 min. The antibacterial activity was expressed as follows: strong (10 mm of inhibition zone between the well and fungal growth); a = week growth (less than < 3 mm); b = medium inhibition (>8 mm); c = strong inhibition (>10 mm of inhibition zone). Means with different letters (a–e) in the same column differ at a p < 0.05 level of probability by the LSD test; ± SD—standard deviation; ** indicates high significance (p < 0.05).