Table 2.
Antibacterial activity of Lp. plantarum strains against Ps. syringae pv. syringae by agar diffusion assay.
LAB Strain | Treatment | |||||
---|---|---|---|---|---|---|
CFS | Trypsin | pH | Proteinase K | Catalase | Boiling | |
Inhibition Zone, mm | ||||||
C6 | 16.00 ± 1.02 d** | 3.10 ± 0.79 c | 1.17 ± 0.36 b | 0.00 a | 0.00 a | 3.03 ± 0.33 c** |
D16 | 23.67 ± 0.93 d** | 0.00 a | 0.00 a | 0.00 a | 18.00 ± 1.36 d** | 0.00 a |
I6 | 16.00 ± 0.85 d** | 0.00 a | 2.03 ± 0.26 d** | 1.67 ± 0.59 b | 0.00 a | 0.00 a |
L1 | 12.17 ± 0.76 c | 26.67 ± 0.92 e** | 0.00 a | 0.00 a | 1.33 ± 0.23 b | 0.00 a |
K1 | 2.33 ± 0.65 a | 0.00 a | 1.63 ± 0.37 c | 0.00 a | 0.00 a | 0.00 a |
Q3 | 15.67 ± 0.83 d** | 5.33 ± 0.46 d** | 1.77 ± 0.59 cd** | 2.50 ± 0.74 c** | 0.00 a | 0.00 a |
Q4 | 23.67 ± 0.98 e** | 3.17 ± 0.85 c | 2.53 ± 0.83 e** | 0.00 a | 2.33 ± 0.47 c** | 1.90 ± 0.42 b** |
S1 | 5.80 ± 0.57 b | 2.00 ± 0.45 b | 2.00 ± 0.72 d** | 0.00 a | 2.23 ± 0.49 c** | 0.00 a |
S4 | 1.70 ± 0.32 a | 0.00 a | 0.00 a | 1.330 ± 0.46 b | 1.70 ± 0.39 bc | 0.00 a |
Note: CFS—cell-free supernatant, Trypsin—CFS + trypsin of 1 mg/mL, pH—pH adjusted from 3.5–4.2 to 6.5, Proteinase K—CFS + proteinase K (100 U/mg), Catalase—CFS + catalase (5 mg), Boiling—boiling of CFS for 20 min. The antibacterial activity was expressed as follows: strong (10 mm of inhibition zone between the well and fungal growth); a = week growth (less than < 3 mm); b = medium inhibition (>8 mm); c = strong inhibition (>10 mm of inhibition zone). Means with different letters (a–e) in the same column differ at a p < 0.05 level of probability by the LSD test; ± SD—standard deviation; ** indicates high significance (p < 0.05).