FIG. 5.

Fimbria-specific CTL in mice immunized with plasmid DNA. Five mice were chosen randomly from each immunized group (day 84), and the pooled splenocytes were restimulated in vitro with fimbriae as effector cells for 4 days at 37°C. Target cells were prepared by adsorption of fimbriae to mastocytoma p815 for 1 h at 37°C. The amount of LDH released upon cell lysis was determined by using CytoTox96. The results express representative data for three experiments.