TABLE 2.
Proliferative responses of splenocytes from mice immunized with DNA vaccinea
| Vaccines used (method) | SI (mean ± SD) |
|---|---|
| pcDNA3/fimA (i.m.) | 9.3 ± 0.34 |
| pcDNA3/fimA (TSG) | 9.5 ± 0.11 |
| pcDNA3 (TSG) | 1.4 ± 0.10 |
| None | 1.4 ± 0.05 |
a
Splenocytes from mice on day 84 were added to 96 wells precoated with fimbria solution for 72 h at 37°C as described in Materials and Methods. During the last 16 h of incubation, 0.5 μCi of [3H]TdR incorporation was determined. The SI was defined as the ratio of counts per minute of stimulated and medium-only cultures. i.m., intramuscular.