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Maximum likelihood phylogenetic tree of the Leishmania ITS1 sequences retrieved from Culicoides biting midges in this study and Genbank, using the JC model. The colored circles indicate our sequences, including L. martiniquensis from six species of Culicoides biting midges (green) and the two recently diagnosed patients (red), and L. orientalis from C. oxystoma and C. peregrinus (yellow). L. infantum isolate RH2 was used as the outgroup.
