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. 2022 Oct 25;14(11):2338. doi: 10.3390/v14112338

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Pathogenicity of NDVs bearing different HN proteins. (A) Clinical signs were observed and recorded once daily. Clinical scores of chickens were calculated according to degree of severity. (B) Clinical symptoms of diseased chickens. rJS/7/05/Ch-infected chickens at 5 dpi (left) and rMukHN494 + 495^JS-infected chickens at 6 dpi (right). Both rJS/7/05/Ch- and rMukHN494 + 495JS-infected chickens showed serious clinical symptoms, including breathing difficulty, lethargy, and decreased appetite. (C) The mRNA levels of the NDV NP gene in peripheral blood of chickens at 1, 3, and 5 dpi. All values were compared with that of rMukteswar. * p < 0.05, ** p < 0.01, and *** p < 0.001.