Table 1.
Primer | Sequence (5′–3′) |
---|---|
HN1 | CGAGCTCGATGGACAGCGCAGTTAGCCAAG (SacI) |
HN2 | CCTCGAGGTTAAACCCCACCATCCTTGAG (XhoI) |
F1 | CCTCGAGGATGATCTGTCTTGATTACTTACAGC (XhoI) |
F2 | GGAATTCCCATTTTTGTAGTAGCTCTCATCTG (EcoRI) |
Sequences shown in bold indicate recognition sites of restriction enzymes.