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Prediction of stabilizing and destabilizing mutations in the HN proteins using the MAESTRO algorithm. ΔΔG_pred, predicted change in free energy (kcal/mol). Negative and positive values indicate a stabilizing and destabilizing mutation, respectively. c_pred, confidence estimation (0–1) of the prediction.
| Mutant | ΔΔG_pred | c_pred |
|---|---|---|
| E495K | −0.12755 | 0.884864 |
| V266I | −0.03536 | 0.86262 |
| A494D | 0.199226 | 0.900809 |
| M145T | 0.019491 | 0.838821 |
