Figure 3.
Effects of C12-cTurg-1, C8-cTurg-2 and chlorhexidine on the kinetics of viability (left) and membrane integrity (right) in B. subtilis 168. Light emission normalized to the untreated water control is plotted as relative luminescence emission (RLU) over time (seconds). Kinetics of the immediate effect (within 3 min) on bacterial viability and membrane integrity, as measured by relative luminescence emission in B. subtilis 168 treated with increasing concentrations of the lipopeptides. Chlorhexidine served as a positive (membranolytic) control and water as a negative (untreated) control. All the graphs of this figure show a representative data set where each experiment was run at least three times independently.