. 2022 Oct 25;12(11):1695. doi: 10.3390/life12111695

Table 1.

List of primers used for the chicken mRNA quantification.

Primer	Sequence 5′–3′	Annealing Temperature/Time	References
IGF1 Fw	GAGCTGGTTGATGCTCTTCAGTT	60 °C/1 min	Xiao et al., 2017 [58]
IGF1 Rev	CCAGCCTCCTCAGGTCACAACT	60 °C/1 min
MYF5 Fw	CAGAGACTCCCCAAAGTGGAGAT	60 °C/1 min
MYF5 Rev	GTCCCGGCAGGTGATAGTAGTTC	60 °C/1 min
PAX7 Fw	AGGCTGACTTCTCCATCTCTCCT	60 °C/1 min	Adhikari et al., 2019 [59]
PAX7 Rev	TGTAACTGGTGGTGCTGTAGGTG	60 °C/1 min	Adhikari et al., 2019 [59]
GAPDH Fw	CCTGCATCTGCCCATTT	59 °C/30 s	De Boever et al., 2008 [60]
GAPDH Rev	GGCACGCCATCACTATC	59 °C/30 s	De Boever et al., 2008 [60]