. 2022 Oct 25;12(11):1695. doi: 10.3390/life12111695

Table 3.

Changes of DNA, RNA, and protein contents and concentrations as well as enzyme activities in pectoralis muscle of control and E. faecium AL41 (EF) supplemented chicks during day 1 to 12 of age.

	Control				EF						p-Value
Day of Age	1 (n = 6)	5 (n = 10)	8 (n = 10)	12 (n = 10)	1 (n = 6)	5 (n = 10)	8 (n = 10)	12 (n = 10)	SE d 1	SE d 5–12	Age	Group	Age × Group
Total DNA, mg	0.48 ^d	3.03 ^c	7.72 ^b	11.38 ^a	0.37 ^d	2.95 ^c	7.58 ^b	12.36 ^a	0.34	0.27	<0.001	0.435	0.123
Total RNA, mg	2.54 ^d	20.42 ^c	48.56 ^b	59.78 ^a,B	2.16 ^d	19.80 ^c	44.34 ^b	67.04 ^a,A	1.96	1.52	<0.001	0.660	0.003
Total protein, mg	80 ^c	501 ^c	2551 ^b	4620 ^a	78 ^c	482 ^c	2753 ^b	4961 ^a	131	102	<0.001	0.098	0.276
DNA/RNA	0.188 ^a,A	0.148 ^b	0.159 ^b,B	0.191 ^a	0.170 ^b,B	0.149 ^c	0.171 ^b,A	0.184 ^a	0.003	0.003	<0.001	0.138	<0.001
RNA/DNA	5.32 ^c,B	6.76 ^a	6.28 ^b,A	5.25 ^c	5.93 ^b,A	6.74 ^a	5.83 ^bc,B	5.44 ^c	0.11	0.09	<0.001	0.249	<0.001
DNA, µg/g	273 ^b,A	366 ^a	217 ^c	195 ^d	226 ^b,B	360 ^a	204 ^c	197 ^c	5	4	<0.001	<0.001	<0.001
RNA, µg/g	1453 ^b	2474 ^a	1362 ^b,A	1024 ^c	1340 ^b	2423 ^a	1192 ^bc,B	1071 ^c	44	34	<0.001	0.008	0.020
Protein, mg/g	45.9 ^d	60.7 ^c	71.6 ^b	79.0 ^a	47.3 ^c	58.8 ^c	74.0 ^b	79.1 ^a	1.6	1.2	<0.001	0.588	0.327
DNA/protein, µg/mg	5.98 ^a,A	6.04 ^a	3.03 ^b	2.46 ^c	4.79 ^b,B	6.13 ^a	2.76 ^c	2.50 ^c	0.12	0.09	<0.001	<0.001	<0.001
RNA/protein, µg/mg	31.8 ^b	40.8 ^a	19.1 ^c,A	12.9 ^d	28.4 ^b	41.3 ^a	16.1 ^c,B	13.6 ^c	0.8	0.6	<0.001	0.009	0.004
Protein/RNA, mg/µg	0.032 ^c	0.025 ^c	0.053 ^b,B	0.077 ^a	0.036 ^c	0.024 ^d	0.063 ^b,A	0.074 ^a	0.002	0.001	<0.001	0.019	<0.001
Total CK, IU	1591 ^c	13,347 ^c	65,827 ^b	116,724 ^a	1612^c	12,557 ^c	67,609 ^b	126,644 ^a	3553	2752	<0.001	0.198	0.218
CK, IU/g	919 ^c	1622 ^b	1845 ^ab	1994 ^a	968 ^c	1540 ^b	1822 ^a	2026 ^a	70	54	<0.001	0.882	0.668
CK/protein, IU/mg	20.2 ^b	26.8 ^a	25.7^a	25.2^a	20.4^b	26.3^a	24.6 ^a	25.7 ^a	1.0	0.8	<0.001	0.703	0.751
ICDH, IU/g	3.12 ^a	2.28 ^b	1.41 ^c	1.25 ^c	2.96 ^a	2.31 ^b	1.39 ^c	1.13 ^c	0.09	0.07	<0.001	0.218	0.533
LDH, IU/g	43 ^d	330 ^c	751 ^b	929 ^a	54 ^d	315 ^c	765 ^b	892 ^a	29	22	<0.001	0.694	0.658
ICDH/protein, IU/mg	0.068 ^a	0.038 ^b	0.020 ^c	0.016 ^c	0.062 ^a	0.039 ^b	0.019 ^c	0.014 ^c	0.001	0.001	<0.001	0.070	0.040
LDH/protein, IU/mg	0.93 ^d	5.42 ^c	10.5 ^b	11.74 ^a	1.14 ^c	5.36 ^b	10.34 ^a	11.28 ^a	0.32	0.25	<0.001	0.536	0.695
LDH/ICDH	14 ^d	146 ^c	540 ^b	748 ^a	19 ^d	138 ^c	554 ^b	796 ^a	29	23	<0.001	0.394	0.660

^a–d Different lower case letters indicate significant differences among ages (p < 0.05). ^A,B Different upper case letters indicate significant differences between groups (p < 0.05).